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Multiple studies using early CRISPR-cas9 agents found that greater than 50% of RNA-guided endonuclease-induced mutations were not occurring on-target. The Cas9 guide RNA (gRNA) recognizes a 20 bp target DNA sequence, which it binds and cleaves to "edit" the DNA sequence. However, target sequence binding can tolerate mismatches up to several ...
50% Off. " 50% Off " is the second episode of the fifth season of the AMC television series Better Call Saul, a spin-off series of Breaking Bad. The episode aired on February 24, 2020, on AMC, in the United States. Outside of the United States, the episode premiered on the streaming service Netflix in several countries.
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